Effects of METAVITAL MNLS technology on cultured connective tissue fibroblasts and inflammatory cells – Metavital

Test report

Effects of METAVITAL MNLS technology on cultured connective tissue fibroblasts and inflammation-mediating cells

The connective tissue forms the supporting structure of the skin and consists of cells (predominantly fibroblasts) and the extracellular matrix of collagen, elastin and proteoglycans. These components give the skin firmness, elasticity and resistance and are important for regeneration, nutrient transport and protection against environmental stresses and influences. Multidimensional non-linear systems (MNLS) are recognized and applied analysis and balancing systems in complementary medicine, which restore the energetic and functional status of an organism. The measurement is based on biophotons. MNLS systems are not yet recognized in conventional medicine.

In this animal-free study with cultivated connective tissue fibroblasts and inflammation-mediating cells (functional neutrophils), the effects of the MNLS-based system from METAVITAL on skin health were investigated. These include an improvement in cell vitality, the promotion of regeneration, the reduction of oxidative stress caused by environmental influences and the reduction of inflammatory processes.

Cell cultures

The investigations were carried out with two different organ-specific cell cultures:

Through an oxidative or respiratory burst, these cells can form superoxide anion radicals locally in the tissue and thus contribute to tissue destruction or an inflammatory process.

The cells of both cell lines were routinelygrown as mass cultures in their special culture media in an incubator at 37 °C in a standardized humid atmosphere of 95 % air and 5 %CO2 and seeded into the corresponding new culture vessels for the tests.

Test product, exposure time and test arrangement

An MNLS-based system from METAVITAL GmbH was made available to us for the duration of the investigations. This was used in accordance with the manufacturer's instructions, with the biophoton trigger sensor consisting of a light intensity sensor and infrared light source positioned 40 mm below the cell culture dishes or bottles (Fig. 1). The exposure was always carried out with 100 % PWM. The signal intensity was between 86 % and 91 % and the reflectance of the signal was always in the green range. The control cells and the treated cells were cultivated in separate mini-incubators at 37 °C in a pH-stable culture medium. The two incubators were placed about 10 meters apart and separated by several house walls. In preliminary cell culture experiments with different exposure times to the infrared light source, we were able to determine a duration of 60 min for an optimal cell effect. Longer exposure times no longer changed the result.

Fig. 1: Experimental set-up with a cell culture multi-plate 40 mm above the biophoton trigger sensor. This arrangement was located in a mini-incubator at a temperature of 37 °C using a pH-stable culture medium. The other devices of the MNLS-based system, such as the METAVITAL box and the notebook with the appropriate software to control the system, are not shown. The wells with the cells are marked by the red culture medium.

Tests performed and results

No further detailed scientific description of the test procedures is provided, as this would impair the general comprehensibility of the test report. If required, this data can of course be supplied at a later date.

4.1 Cell vitality

Cell vitality and cell metabolism are fundamental for the function and adaptability of tissues: Metabolism provides the energy and building materials necessary for growth, differentiation, repair and signal transmission. This promotes cell vitality and cell health. Connective tissue fibroblasts were seeded into the wells of a multiwell culture plate and, after settling and spreading for 24 hours and resuming cell metabolism, treated with the MNLS-based system for 60 minutes and then cultured for a further 24 hours. The control cells remained untreated. The activity of mitochondrial dehydrogenases was then quantified using an enzyme assay (XTT test). An experiment was carried out with several replicates.

Result: A single exposure to the MNLS-based system from METAVITAL improved the vitality of the connective tissue fibroblasts by around 10 % compared to the untreated control. This demonstrates the potential of the MNLS-based system for this cell type as well, as the observed effect can possibly be significantly enhanced by several consecutive exposures.

4.2 Regeneration

Cell regeneration is a fundamental biological process that enables organisms to replace damaged or dead cells and thus maintain homeostasis. By promoting regeneration, the integrity and functionality of the affected tissue area can be restored earlier. In this test, the colonization of a cell-free space after 12 hours was determined as a measure of the cell regenerative potential when treated with the MNLS system for one hour directly at the beginning of the regeneration phase. The control cells remained untreated. At the end of the experiment, the remaining uncolonized area was documented using series of photomicrographs and evaluated using special AI software. An experiment was carried out with several replicates.

Result: The residual area at the end of the trial was 7 % of the total area for the treated cells and over 9 % for the untreated cells. This corresponds to an improvement of around 20 % as a result of the single treatment with the MNLS-based system from METAVITAL (Fig. 2).

In this expert interview, Prof. Dr Peter C. Dartsch talks to Sophia about the latest cell biology research into METAVITAL’s MNLS technology.

Key findings:

Improved cell vitality
Significant increase in regeneration
Protection against oxidative stress – and all this after just a single exposure of the cells.

Particularly exciting:

The studies show that technologies such as MNLS and biophoton technology can have measurable effects at the cellular level.

Outlook for the future:

Prof. Dr Dartsch sees the potential for conventional medicine and METAVITAL technology to work together over the next 10–20 years.

Fig. 2: Photomicrograph documentation of the cell-free space remaining after 12 hours in the untreated control cells (A) compared to the treated cells (B).

4.3 Environmental oxidative stress

After 24 hours of exposure to different concentrations of hydrogen peroxide (0.5 to 1.5 mM) as a donor for reactive oxygen species in the culture medium, the viability of the connective tissue fibroblasts was examined with and without an initial one-hour treatment with the MNLS-based system from METAVITAL . This simulated a situation in which external influences can lead to undesirable oxidative stress with the resulting health consequences for the skin, such as reduced barrier function, irritation, etc. Viability was measured using an enzymatic test. An experiment was carried out with several replicates.

Result: The viability of the cells decreased with increasing hydrogen peroxide concentration in the culture medium. At 1.5 mM hydrogen peroxide, the viability for the treated connective tissue fibroblasts was 74 % and for the control cells only 52 %. The difference was therefore more than 20 %. At the lower hydrogen peroxide concentrations with much higher survival rates above 85 %, the difference between the treated and untreated cells was significantly smaller at a maximum of 5 %.

4.4 Endogenous radical formation in tissue

In most mammals, neutrophils are the most common type of granulocyte, i.e. a specific type of white blood cell. They play a role as phagocytes (= scavenger cells) in the blood as a cellular defense against microbial foreign germs and – after immigration into the tissue – as inflammation-mediating cells. An oxidative burst of the cells after stimulation can lead to the increased formation of reactive radicals, which kill the foreign germs in the blood and then remove them by phagocytosis, but can lead to the triggering of inflammatory processes in the tissue. The promyelocytes were differentiated into functional neutrophils by adding 1.5 % dimethyl sulfoxide for 6 days. On the last two days of differentiation, the cells were treated for one hour each with the MNLS-based system from METAVITAL. The control cells remained untreated. Afterwards, their inactivation was quantitatively determined by cleavage of a dye by the radicals formed in the oxidative burst. Four independent experiments were performed with replicates.

Result: Treatment with the MNLS-based system from METAVITAL resulted in a stronger and statistically significant reduction in endogenous radical formation in the tissue by 18.8 ± 4.4 % compared to untreated controls (mean values ± standard deviations; p ≤ 0.01; two-sided Wilcoxon-Mann-Whitney rank sum test). This is characteristic of the inhibition of inflammatory processes by inactivating the superoxide anion radicals formed by the cells.

Oxidative stress from the environment and induction of a neurodegenerative process: protective effect of treatment with the MNLS-based system from METAVITAL

The survivability of the cultured neuronal cells after an artificially induced oxidative stress in the environment of the cells was continuously recorded under the microscope. For this purpose, reactive oxygen species (ROS) were added to the culture medium. An untreated culture served as a direct comparison.

One image was recorded in 30 seconds and later every second image was taken from the series and put together to form a time-lapse video clip. This means that one image per minute was used for the clip. When playing back 25 images per second, this means a time lapse by a factor of 1,500 x, i.e. one second in the time-lapse video corresponds to 25 minutes in reality. The same procedure was used with the untreated control culture.

It is clearly recognizable that exposure to the MNLS-based system leads to a protective effect against the ROS and that far fewer neuronal cells than in the untreated control initially round off, detach from the culture dish and eventually die.

These time-lapse video clips thus document the protective effect of METAVITAL’s MNLS-based system in dynamic form. This effect can result in reduced oxidative stress and thus also a reduction in neurodegenerative processes in the body.

Conclusion

As the studies presented here have shown at the cellular level, METAVITAL 's MNLS-based system has beneficial properties that can also improve and maintain skin health in a complex whole organism by promoting the basic properties of the fibroblasts of the connective tissue.

Responsible for the scientific accuracy of the tests performed and the content of the test report.

Materials

Effects of METAVITAL MNLS technology on cultured connective tissue fibroblasts and inflammation-mediating cells

Test report
dartSCIENTIFIC

Dießen am Ammersee, 18.01.2026